LncRNA EPR transcriptional activity controls intestinal mucus and prevents susceptibility to inflammation and tumorigenesis [RNA-seq]

The lncRNA EPR (a.k.a BC030870) is highly enriched in the gastrointestinal tract in mice and, more specifically, in the large intestine. We generated conditional EPR knock-out in large intestine and analyzed genome-wide the gene expression changes by RNA-Seq. Total RNA was extracted from the proximal colon of six control (EPR fl/fl) and six knock-out (EPR cKO) mice. Libraries were constructed and sequenced. Results indicate that EPR knock-out predominantly leads to down-regulation of genes implicated in mucus biogenesis. These results help in explaining the phenotype displayed by EPR cKO mice that is characterized by higher susceptibility to intestinal inflammation and cancer formation. Total RNA was extracted from the proximal colon of six control (EPR fl/fl) and six knock-out (EPR cKO) mice.长链非编码RNA（long non-coding RNA, lncRNA）EPR（亦称BC030870）在小鼠胃肠道中高度富集，且在大肠中富集程度尤为显著。我们在大肠中构建了EPR的条件性敲除模型，并通过RNA测序（RNA-Seq）对全基因组范围的基因表达变化进行了分析。我们从6只对照小鼠（EPR fl/fl）及6只EPR条件性敲除（EPR cKO）小鼠的近端结肠中提取了总RNA。构建测序文库并进行测序。结果显示，EPR敲除主要导致参与黏液生物合成的基因表达下调。该结果有助于解释EPR cKO小鼠所表现出的表型——这类小鼠对肠道炎症及肿瘤发生具有更高的易感性。我们从6只对照小鼠（EPR fl/fl）及6只EPR条件性敲除（EPR cKO）小鼠的近端结肠中提取了总RNA。