Bidens pilosa L. (Asteraceae) cultivated in Brazil on acute liver disease in dogs

ABSTRACT Bidens pilosa L. is a medicinal plant popularly used for treatment of liver diseases. In this study, the dry extract of aerial parts of Bidens pilosa and Silymarin, a phytocomplex obtained from the Silybum marianum fruits and marketed as hepatoprotective, were tested in dogs experimentally acutely intoxicated with carbon tetrachloride. The liver activity was evaluated by hematological and biochemical profiles, and histological and ultrasound analyzes. It was observed that the lowest serum activities of ALT and serum concentrations of total bilirubin occurred in the groups treated with the dry extract of Bidens pilosa, while only decreased serum concentrations of total bilirubin occurred in the group treated with Silymarin. Best liver recovery was also observed for the dry extract of B. pilosa at a 400mg/Kg dose by ultrasonography. This study showed that the dry extract of Bidens pilosa acted more efficiently in the treatment of acute toxic hepatitis induced in dogs than Silymarin.摘要 三叶鬼针草（Bidens pilosa L.）是一类常用于肝脏疾病治疗的药用植物。本研究以从水飞蓟（Silybum marianum）果实中提取、作为保肝药物上市的植物复合物水飞蓟素（Silymarin），与三叶鬼针草地上部分干提取物为受试物，在经四氯化碳（carbon tetrachloride）实验性急性中毒的犬只中开展药效评价。研究通过血液学与生化指标谱检测、组织病理学分析及超声检查，对受试犬的肝脏功能状态进行评估。结果显示，给予三叶鬼针草地上部分干提取物的各组血清丙氨酸氨基转移酶（ALT）活性与血清总胆红素浓度均处于最低水平；而仅水飞蓟素给药组的血清总胆红素浓度出现显著降低。超声检查结果表明，以400mg/kg剂量给药的三叶鬼针草干提取物组，其肝脏恢复效果最佳。本研究证实，三叶鬼针草干提取物在治疗犬四氯化碳诱导的急性中毒性肝炎方面，疗效优于水飞蓟素。