Transcriptomic profiling of murine adipose endothelial cells from high-fat diet fed mice

Female mice display greater adipose angiogenesis and maintain healthier adipose tissue than do males upon sustained high-fat diet feeding. We utilized transcriptome analysis of endothelial cells (EC) from the white adipose tissue of male and female mice that were fed a high-fat for 7 weeks. We found that adipose EC exhibited pronouncedly sex-distinct transcriptomes. Genes upregulated in female adipose EC were associated with proliferation, oxidative phosphorylation, and chromatin remodeling contrasting the dominant enrichment for genes related to inflammation and a senescence-associated secretory of male EC. Our study provides novel insights into molecular programs that distinguish male and female EC responses to pathophysiological conditions. Overall design: Male and female mice were fed a high fat diet for 7 weeks, starting at 7 weeks of age. Endothelial cells were purified from the white adipose tissues of these mice and RNA was extracted and analyzed by RNA-sequencing.