Figures 28-40 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 28-40. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Growth Phase. Figure 28 Oocyte during late vitellogenesis filled with yolk granules (Y) and lipid droplets (Li). Note the cytoplasmic patches where the annulate lamellae (Al) are located and the welldeveloped egg envelope (Eg) (810x). Figure 29 Ripe oocytes occupy the entire coelomic cavity toward the body wall (810x). MC = muscle; Ep = epidermis. Figure 30 General view of the annulate lamellae (Al) between yolk granules and lipid droplets (560X). Figures 31-33 The annulate lamellae are composed by fenestrated endomembranes (arrows) parallel to each other arranged (Figure 31, 2,900X. Figure 32, 10,500X. Figure 33, 3,100X). Figure 34 Mature yolk granules with an elliptical shape (Y) showing a medullar crystalline substructure (white arrow) and non-crystalline cortex with electron-lucent spheres (black arrow) (13500X). Figure 35 Crystallised medullar in a parallel arrangement (white arrow) and a cortical lucid sphere (black arrow) (43,000X). Figure 36 Yolk granules (Y) and lipid droplets (Li) in the cytoplasm, with the presence of glycogen granules (arrow) close to the droplets (13,500X). M = mitochondria. Figure 37 Details of yolk granules delimited by the membrane (arrow) next to a lipid droplet (Li) and glycogen (Gly) (61000X). Figure 38 Cortical cytoplasm filled with yolk granules (Y), lipid droplets (Li) and cortical granules (CG) below the plasma membrane. Notice the thick egg envelope (Eg) (2,050X). Figure 39 The perivitelline space (1) is narrow and the egg envelope (Eg) is composed of two layers (2 and 3) with different electron-densities. The winding microvilli (Mv) are completely immersed in the egg envelope. Only the apical surface of the microvilli is in contact with the coelomic cavity (arrow) (5,400X). CG = cortical granules. Figure 40 Microvilli apex showing the expansion bears extensive filamentous adornment with an electron-dense centre (31,000X).图28~40 西南大西洋产尾叶缨鳃虫（Phragmatopoma caudata）的超微结构：生长阶段 图28 卵黄发生晚期的卵母细胞，其内充满卵黄颗粒（yolk granules，Y）与脂滴（lipid droplets，Li）。可见环孔片层（annulate lamellae，Al）所在的胞质区域，以及发育良好的卵被膜（egg envelope，Eg）（放大倍数810×）。 图29 成熟卵母细胞占据朝向体壁的整个体腔（放大倍数810×）。MC=肌肉（muscle）；Ep=表皮（epidermis）。 图30 卵黄颗粒（Y）与脂滴（Li）之间环孔片层（Al）的全貌（放大倍数560×）。 图31~33 环孔片层（Al）由彼此平行排列的有窗孔内膜（fenestrated endomembranes）构成（箭头指示）（图31：2900×；图32：10500×；图33：3100×）。 图34 成熟卵黄颗粒（Y）呈椭圆形，具有髓质晶体亚结构（白色箭头指示），以及带有电子透亮球体的非晶质皮质层（黑色箭头指示）（放大倍数13500×）。 图35 呈平行排列的结晶状髓质（白色箭头指示）与皮质透亮球体（黑色箭头指示）（放大倍数43000×）。 图36 胞质中的卵黄颗粒（Y）与脂滴（Li），脂滴附近可见糖原颗粒（glycogen granules，箭头指示）（放大倍数13500×）。M=线粒体（mitochondria）。 图37 被膜包被的卵黄颗粒细节（箭头指示），其旁可见脂滴（Li）与糖原（glycogen，Gly）（放大倍数61000×）。 图38 质膜下方的皮质胞质中充满卵黄颗粒（Y）、脂滴（Li）与皮质颗粒（cortical granules，CG）。可见厚实的卵被膜（Eg）（放大倍数2050×）。 图39 狭窄的卵周隙（perivitelline space，1），卵被膜（Eg）由两层电子密度不同的结构（2与3）构成。缠绕的微绒毛（microvilli，Mv）完全嵌入卵被膜中，仅微绒毛的顶端表面与体腔接触（箭头指示）（放大倍数5400×）。CG=皮质颗粒。 图40 微绒毛（Mv）顶端可见膨大结构，带有大量丝状附属物，中心具有电子致密区域（放大倍数31000×）。