The Effect of Kuntai Capsule on Ovarian Function in Cisplatin-Induced Premature Ovarian Insufficiency Rats

Abstract <strong>Objective</strong>: This study aims to evaluate the effect of the Kuntai Capsule on ovarian function in cisplatin-induced premature ovarian insufficiency (POI) rats and to explore the mechanism of the Kuntai Capsule on the ovarian function of rats. <strong>Methods</strong>: Seventy-four female Sprague-Dawley rats were used for this study. Eight of the rats were randomly assigned to the Control group. The remaining sixty-six rats were utilized to establish the POI model via Cisplatin and then randomly divided into four groups: the model Control group (mo Control), the Estradiol group (E2), and the low and high doses of Kuntai Capsule groups (KTC-L, KTC-H). For the 28-day administration, the Control and mo Control groups were intragastrically administered with 2.0 mL of 0.9% sodium chloride daily, the E2 group with 2.0 mL of Estradiol suspension (0.2mg/kg/d), and the KTC-L and KTC-H groups with 2.0 mL of Kuntai capsule suspension (0.6g/kg/d, 1.8g/kg/d, respectively). Sex hormone levels, estrous cycle, and ovarian coefficient of the five groups were compared, histological sections analyzed follicle counts, and the protein expressions of GDF9, LC3A-II, and Beclin 1 in the ovarian tissue were detected by Western blotting. <strong>Results</strong>: After the 28-day administration, the E2, FSH levels of the KTC-L group was not significantly different with the Control group, the AMH level of the KTC-H group was significantly higher than the E2 group. The estrous cycle of KTC-L was significantly lower than the mo Control group. Regarding ovarian coefficient, resting and growing follicles, and GDF9, LC3A-II, and Beclin 1 expression, both KTC groups outperformed the mo Control group with the statistical difference (<em>P</em>&lt;0.05). <strong>Conclusion</strong>: Kuntai Capsule can improve POI rats’ estrous cycle and ovarian coefficient, maintain the number of resting and growing follicles, and up-regulate the protein expression of GDF9, LC3A-II, and Beclin 1 of rats’ ovaries.**摘要** **研究目的**：本研究旨在评估坤泰胶囊对顺铂诱导的早发性卵巢功能不全（premature ovarian insufficiency, POI）模型大鼠的卵巢功能影响，并探讨坤泰胶囊对大鼠卵巢功能的作用机制。 **研究方法**：本研究共使用74只雌性斯普拉格-道利（Sprague-Dawley, SD）大鼠，其中8只随机分为空白对照组。剩余66只大鼠通过顺铂构建早发性卵巢功能不全模型，随后随机分为4组：模型对照组（mo Control）、雌二醇组（Estradiol, E2）、低剂量坤泰胶囊组（KTC-L）与高剂量坤泰胶囊组（KTC-H）。给药周期为28天：空白对照组与模型对照组每日灌胃2.0 mL 0.9%氯化钠溶液；E2组每日灌胃2.0 mL雌二醇混悬液（0.2mg/kg/d）；KTC-L与KTC-H组每日灌胃2.0 mL坤泰胶囊混悬液，剂量分别为0.6g/kg/d与1.8g/kg/d。本研究对5组大鼠的性激素水平、动情周期及卵巢系数进行比较，通过卵巢组织切片计数卵泡数量，并采用蛋白质免疫印迹（Western blotting）检测卵巢组织中生长分化因子9（Growth Differentiation Factor 9, GDF9）、LC3A-II及Beclin 1的蛋白表达水平。 **研究结果**：给药28天后，KTC-L组的雌二醇与卵泡刺激素（Follicle-Stimulating Hormone, FSH）水平与空白对照组无显著差异；KTC-H组的抗缪勒管激素（Anti-Müllerian Hormone, AMH）水平显著高于E2组。KTC-L组的动情周期显著短于模型对照组。在卵巢系数、静息卵泡与生长卵泡数量，以及GDF9、LC3A-II及Beclin 1的蛋白表达方面，两个坤泰胶囊给药组均优于模型对照组，差异具有统计学意义（P<0.05）。 **研究结论**：坤泰胶囊可改善顺铂诱导的早发性卵巢功能不全模型大鼠的动情周期与卵巢系数，维持静息卵泡与生长卵泡数量，并上调大鼠卵巢组织中GDF9、LC3A-II及Beclin 1的蛋白表达水平。