Expression data from neural stem cells treated with endotherial progenitor-derived soluble protein. Mus musculus

Endogenous neural stem cells reside in the mammalian brain during development and within special niche microenvironments during adulthood. We discovered that endothelial progenitor cell-secreted factors promote the self-renewal of adult mouse NSCs in vivo and identified this protein as ECF-L. We also analyzed molecular signalling networks caused by ECF-L in neural stem cells using global gene expression analysis. Overall design: Neural stem cells were isolated and expanded from adult mouse brain. Neural stem cells were cultured in the presence or absence of ECF-L in the MHM medium. After the designed period of ECF-L treatment, cells were harvested and RNA was extacted, followed by DNA microarray analysis.