Single cell RNA sequencing of lineage traced early postnatal myofibroblasts in the adult lung. Single cell RNA sequencing of lineage traced early postnatal myofibroblasts in the adult lung

Early postnatal myofibroblasts are a key cell type involved in postnatal alveolarization. The majority of these cells undergo apoptosis post-alveolarization. However, some dedifferntaite and persist in the adult lung. We utilized single cell RNA seq. to understand transcriptomics of these persisted myofibroblasts. Overall design: Acta2-CreERT2, ROSA26R(ZSgreen/+) mice were injected with 4-OH-TM on P6.5, P8.5 and P10.5, and at P60.5, single cell suspension of the lungs were made. The FACS sorted, ZSgreen+, cells were subjected to single cell RNA sequencing to assess marker expression in ZSgreen+ cells that were also SMA+ or SMA