A critical role of fibroblast in cardiac maturation as revealed by single-cell RNA-Sequencing

The mammalian postnatal heart undergoes orchestrated and systematic changes to culminate in functional maturation, yet little is known about the cellular and molecular foundations underlying these processes. Here, we reported a critical role of fibroblast in cardiac maturation as revealed by single-cell RNA-Sequencing, both in vivo and in vitro.We also identified the key ligands secreted by fibroblast that are involved in the regulation of cardiac maturation. RNA-Seq of mouse neonatal cardiomyocytes co-cultured with neonatal fibroblasts or adult fibroblasts; Single cell RNA-Seq of co-culture system of cardiomyocytes and fibroblasts