miRNA Nanostring Data on a Silk Scaffold model following CCI injury

This study investigates the effects of controlled cortical impact (CCI), a form of traumatic brain injury (TBI), using a 3D silk scaffold cell culture model. Human induced neural stem cells (iNSCs), human induced astrocytes, and HMC3 microglial cells were seeded onto the silk scaffolds and allowed to grow under controlled conditions. A subset of these cell cultures was then subjected to CCI to simulate injury. The primary aim of the study was to assess the cellular and molecular response to CCI, particularly focusing on miRNA expression profiles. miRNA Nanostring technology was employed to quantify differential expression patterns associated with CCI exposure, providing insights into neuroinflammatory, regenerative, and apoptotic pathways impacted by the injury. These data contribute to a better understanding of miRNA's role as a biomarker for TBI and its potential involvement in cellular recovery and neuroprotection processes following traumatic injury. Each scaffold is one sample. Each independent experiment consisted of 36 samples. 6 sham (no CCI) and 6 CCI samples had media collected for miRNA analysis at the 3 timepoints assessed in this study (6, 24, and 72 hours). Thus, 12 samples were assessed at 6 hours, 12 at 24 hours, and 12 at 72 hours within Experiment #1. 2 independent experiments were used to generate this data. Both experiments together included 72 samples, 24 per timepoint.