scRNAseq of PBMCs from idiopathic multicentric Castleman Disease (iMCD) flare and remission

We performed scRNAseq of PBMCs from three idiopathic multicentric Castleman Disease (iMCD) patients with paired flare and remission samples 6 PBMC samples were analyzed, 3 from flare and three from remission, with two technical replicates. Cryopreserved PBMCs were thawed and immediately loaded on to a GemCode instrument (10X Genomics) to produce single-cell bar-coded droplets using 10X Single Cell 3’ v2 chemistry. Libraries generated were sequenced across at least one lane on the Illumina HiSeq2500 instrument with the HiSeq Rapid SBS kit.