Histone demethylase SlJMJ7 Integrates DNA demethylation, ethylene signal and major ripening-related transcription factors in coordination of fruit ripening in tomato [ChIP-seq]

Combinational analyses of genome-wide transcription, binding sites and histone H3K4 trimethylation profiles revealed that SlJMJ7 directly binds and represses the expression of a series of key ripening-associated factors, including ethylene biosynthesis genes ACS2/4/8 and ACO2, DNA demethylase DML2, as well as the ripening-related transcription factors RIN and NOR by removal the mark of H3K4-trimethylation. Moreover, SlJMJ7 directly and indirectly modulates the transcription of genes related to diverse physiological traits of fruit ripening. Overall design: Tomato histone modification H3K4me3 profiles of wild type (WT) and transgenic knockout of JMJ7 lines (jmj7) at 38 days after anthesis. JMJ7 target gene identification sample is JMJ7-OE-GFP tomato pericarp at 38 days after anthesis.