Gene expression profile of bone marrow cells from leukemic mice by transduction of synonymous mutant of MLL-AF4

The establishment of a murine disease model of MLL-AF4 via retroviral transduction has been difficult due to a lack of understanding of its unknown regulatory mechanisms. We showed that MLL-AF4 protein is post-transcriptionally regulated, and its synonymous mutant is able to induce myeloid leukemia by retroviral transduction. To validate synonymous mutant of MLL-AF4 is phenotypically similar to the MLL-mAf4, we analyzed the gene expression profiles by RNA-seq. We purified RNA from bone marrow cells when the sign of leukemia is observed and performed gene expression profiling analysis by RNA-seq.