Transcriptomic analysis of gill tissues in blunt snout bream (Megalobrama amblycephala) in response to bacterial infections under hypoxia

This study aims to characterize the transcriptomic responses of M. amblycephala exposed to A. hydrophila under hypoxia. First, we conducted low-oxygen and immune assessments for different groups of fish to determine the differences in antioxidant capacity and immune function between resistant and sensitive fish. Next, we performed sequencing analysis to identify the genes that exhibit these differences, and subsequently, we validated the sequencing results using quantitative PCR (qPCR).