Table listing the lowest detection level within the linear response range for one peptide per protein in the 11-plex AFFIRM assay in the three different magnetic bead systems.

The assay for each target protein in the three systems is rated into three grades of assays, A-C. Rating is based on lowest detected concentration in the linear response range where linear response is defined by an R2 value above 0.9. The assay grades are defined as; A = 0.05–1 ng/ml; B = 5–12.5 ng/ml; C = 25+ ng/ml of lowest detected peptide level in the linear response range. This detection level is combined with the R2 value where the assay grade is downgraded one level (i.e. from A to B or from B to C) if the R2 value is below 0.9. The lowest concentration of detected target proteins within the linear response is displayed as both ng/ml and fmol/ml for respective bead system. The epoxy system could only detect 6 out of the 11 proteins.