Innate Lymphoid Cells-Containing Fraction of Murine Lamina Propria Cells Produces Interleukin-17 upon Incubation with Giardia lamblia

Innate lymphoid cells (ILCs) are key players during an immune response at the mucosal surfaces, such as lung, skin, and gastrointestinal tract. Giardia lamblia is an extracellular protozoan pathogen that inhabits the human small intestine. In this study, an ILC-containing fraction (ICF) were prepared from the lamina propria of murine small intestine and incubated with G. lamblia trophozoites in the presence of transwell membranes. Transcriptional changes in Giardia-exposed ICFs were examined by RNA-seq analysis, resulting in identification of activation of several immune pathways. Overall design: mRNA expression profiles of the mouse ILC-containing fraction (ICF) only cells or G. labmlia incubated ICF cells by Illumina Hi-seq 4000.