RNA-seq of small cell lung cancer [circRNA-Seq]. RNA-seq of small cell lung cancer [circRNA-Seq]

We used CCK-8 experiment to determine the chemotherapy tolerance of small cell lung cancer cells, from which we found chemotherapy-sensitive cells (H446) and chemotherapy-tolerant cells (SHP77).By RNA-SEQ, we identified 401 circRNAs with abnormal expression associated with chemotherapy tolerance, including 212 up-regulated circRNAs and 189 down-regulated circRNAs. Through qRT-PCR verification, several circRNAs related to chemotherapy tolerance of small cell lung cancer were found, which helped us to further clarify the mechanism of chemotherapy tolerance of small cell lung cancer. Overall design: CircRNA profiles of small cell lung cancer