湿加松体细胞胚拯救的方法

本发明涉及一种湿加松体细胞胚拯救的方法,包括(1)采集湿加松球果、获取外植体;(2)诱导产生胚性愈伤组织:(3)胚性愈伤组织的增殖:(4)熟化培养获得具有萌发潜力的子叶胚;(5)萌发培养获得萌发苗的步骤;所述采集湿加松球果的时间为6月20日-7月2日;所述诱导培养基中的激素组分包括:2-2.4μM的6-苄氨基嘌呤、0.008-0.012μM的芸苔素内酯、7-7.4μM的2,4- D丁酯。该方法具有较高的胚性愈伤组织的诱导率、体胚成熟率以及萌发率。This invention relates to a method for somatic embryo rescue of Pinus elliottii × Pinus caribaea. The method includes the following steps: (1) Collecting cones of Pinus elliottii × Pinus caribaea and acquiring explants; (2) Inducing embryogenic callus formation; (3) Proliferating embryogenic callus; (4) Carrying out maturation culture to obtain cotyledonary embryos with germination potential; (5) Performing germination culture to obtain germinated seedlings. The timing for collecting Pinus elliottii × Pinus caribaea cones is from June 20 to July 2. The hormone components in the induction medium are: 2–2.4 μM 6-benzylaminopurine, 0.008–0.012 μM brassinolide, and 7–7.4 μM 2,4-D butylate. This method exhibits relatively high induction rate of embryogenic callus, somatic embryo maturation rate and germination rate.

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背景与挑战

背景概述

该数据集描述了一种湿加松体细胞胚拯救的方法，涵盖从外植体采集到萌发苗培养的完整步骤，旨在提高胚性愈伤组织的诱导率、体胚成熟率和萌发率。它属于'油松等速生建筑材林高效培育技术研究'项目，以文档格式提供，为协议共享数据。