Data from: Systems and trans-system level analysis identifies conserved iron deficiency responses in the plant lineage

We surveyed the iron nutrition-responsive transcriptome of Chlamydomonas
reinhardtii using RNA-Seq methodology. Presumed primary targets were
identified in comparisons between visually asymptomatic iron-deficient
versus iron-replete cells. This includes the known components of
high-affinity iron uptake as well as candidates for distributive iron
transport in C. reinhardtii. Comparison of growth-inhibited iron-limited
versus iron-replete cells revealed changes in the expression of genes in
chloroplastic oxidative stress response pathways, among hundreds of other
genes. The output from the transcriptome was validated at multiple levels:
by quantitative RT-PCR for assessing the data analysis pipeline, by
quantitative proteomics for assessing the impact of changes in RNA
abundance on the proteome, and by cross-species comparison for identifying
conserved or universal response pathways. In addition, we assessed the
functional importance of three target genes, VITAMIN C 2 (VTC2),
MONODEHYDROASCORBATE REDUCTASE 1 (MDAR1), and CONSERVED IN THE GREEN
LINEAGE AND DIATOMS 27 (CGLD27), by biochemistry or reverse genetics. VTC2
and MDAR1, which are key enzymes in de novo ascorbate synthesis and
ascorbate recycling, respectively, are likely responsible for the 10-fold
increase in ascorbate content of iron-limited cells. CGLD27/At5g67370 is a
highly conserved, presumed chloroplast-localized pioneer protein and is
important for growth of Arabidopsis thaliana in low iron.