EcoliOverExpressionDB

Recombinant protein production is a significant biotechnological process as it allows researchers to produce a specific protein in desired quantities. Escherichia coli ( E. coli ) is the most popular heterologous expression host for the production of recombinant proteins due to its advantages such as low cost, high-productivity, well-characterized genetics, simple growth requirements and rapid growth. There are a number of factors that influence the expression level of a recombinant protein in E. coli which are the gene to be expressed, the expression vector, the expression host, and the culture condition. The major motivation to develop our database, EcoliOverExpressionDB, is to provide a means for researchers to quickly locate key factors in the overexpression of certain proteins. Such information would be a useful guide for the overexpression of similar proteins in E. coli. To the best of the present researchers’ knowledge, in general and specifically in E. coli, EcoliOverExpressionDB is the first database of recombinant protein expression experiments which gathers the influential parameters on protein overexpression a nd the results in one place.重组蛋白生产是一项具有重要意义的生物技术工艺，可帮助研究人员按照预期产量制备目标蛋白质。大肠杆菌（Escherichia coli，下称E. coli）是目前应用最广泛的重组蛋白异源表达宿主，其具备成本低廉、表达产量高、遗传特性已被充分解析、培养条件简单且生长速度快等诸多优势。影响大肠杆菌重组蛋白表达水平的因素众多，主要涵盖待表达基因、表达载体、表达宿主及培养条件四大类。本团队开发EcoliOverExpressionDB数据库的核心动机，在于为研究人员提供快速检索特定蛋白质过量表达关键影响因素的途径。此类信息可为大肠杆菌体系中同类蛋白质的过量表达研究提供有效参考。据目前所知，在重组蛋白表达领域，尤其是大肠杆菌表达体系中，EcoliOverExpressionDB是首个将蛋白质过量表达的影响参数与实验结果整合收录的重组蛋白表达实验数据库。

搜集汇总

背景与挑战

背景概述

EcoliOverExpressionDB是一个专注于大肠杆菌重组蛋白过表达的数据集，旨在收集和整合影响蛋白表达的关键实验参数（如基因、载体、宿主和培养条件）及其结果，为研究人员提供快速参考和指导，以优化类似蛋白的表达过程。这是该领域首个此类数据库，具有实用性和创新性。