Antibiotics Experiment - Cell sorting

Flow cytometry data on mouse fecal bacteria incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, in a 1:1 ratio of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating bacteria. Bacteria were exposed to Polymyxin B (all samples with "P"), Vancomycin ("V"), or the vehicle (DMSO; "C") for one hour before incubation with EdU. The antibiotics had a final concentration of 0.1 mg.mL-1. The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.