FGF4 stimulation increases variability in mESCs [scRNA-seq]. FGF4 stimulation increases variability in mESCs [scRNA-seq]

FGF4 is essential for Epiblast and Primitive Endoderm formation in the mouse embryo and promotes differentiation of mouse embryonic stem cells. However, different FGF-concentrations regulate gene expression quantitatively remained an open question. We used single-cell RNA sequencing to quantify transcriptional variability and dynamics upon FGF4 stimulation of Fgf4-mutant mESCs. Overall design: Fgf4-mutant mouse embryonic stem cells in N2B27 + Chiron were stimulated for 24 h with different concentration of FGF4.