wt_perfusion_fixed

Tilt Series Date: 2015-09-16 Data Taken By: Poorna Subramanian Species / Specimen: Shewanella oneidensis Strain: MR-1 Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 41000x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: The cells look good on scope. Next time use 1.5ul gold. Collaborators and Roles: Moh and Sahand USC Purification / Growth Conditions / Treatment: This sample is wt cells grown in perfusion set-up. It is fixed with 2.5% glutaraldehyde for 15 minutes. The grid was prepared by Sahand on 9/10/15. The grid was triple rinsed in distilled water and saved in distilled water until freezing on 9/15/15 at USC using manual plunger. Sample Preparation: This is gold grid with honey C. The grid was pulled out quickly from media. 1.2ul of 10nm gold was added immediately to the cells on the C side -same side as cells were present. The grid was loaded on manual plunger and blotted from behind- opposite of C side. And then plunge frozen.