mRNA bisulfite sequencing

Recent studies suggest that DNA damage-induced RNA modifications, including the m5C modification, promote TC-HR and contribute to cancer cell survival. However, the distribution of m5C in the genome remains elusive. Furthermore, whether m5C formation has any sequence or structural preferences has not been studied at a genome scale. To determine the distribution of TRDMT1-mediated m5C modification in the genome, we used the mRNA isolated from U2OS WT and TRDMT1 KO cells to perform bisulfite sequencing. Because TRDMT1-mediated m5C formation is stimulated by DNA damage, cells were treated H2O2 to enhance the modification.