Replication Data for: A pilot study on midazolam sedation for murine echocardiography: A potential alternative to isoflurane anesthesia and awake imaging

Data described in the manuscript - Isoflurane anesthesia is often used to facilitate murine echocardiography, butcan suppress cardiac function. Awake imaging avoids pharmacological inter-ference, but can induce sympathetic activation. In this Midazolam sedation.Parameters were compared using repeated-measures ANOVA. Midazolam ena-bled imaging without overt stress behavior. Compared to midazolam, heart ratewas similar under isoflurane and higher while awake (p ≤ 0.01). End-systolicvolume was larger under isoflurane and smaller while awake; stroke volumesremained similar across conditions. Global longitudinal and circumferentialstrain were less negative under isoflurane (p = 0.03) but similar during awakeimaging, while radial strain was higher during awake imaging. Peak longitudi-nal strain rate was less negative under isoflurane (p ≤ 0.01) and more negativewhile awake (p = 0.05). Early diastolic strain rate was similar under isofluraneand lower while awake (p = 0.02). In conclusion, cardiac function was mostdepressed under isoflurane and most enhanced during awake imaging, likelystress-driven. Murine echocardiography under midazolam sedation was feasi-ble, yielding better function than isoflurane anesthesia, closer to awake imag-ing but without overt handling stress. These findings require further validationacross disease models, sexes, and strains.