Functional effects on HUVEC of CD63+ and MHC class 1+ subsets of extracellular vesicle produced by wild type and CD47-deficient T cells

The present study revealed that CD63+ and MHC-1+ EVs from CD47-deficient T cells are enriched in small non-coding RNAs relative to the respective EVs from WT cells. CD47-deficient T cells secrete more CD63+ and MHC-1+ EVs, but MHC-1+ EVs are selectively taken up more by human umbilical vein endothelial cells. Microarray analysis of endothelial cells treated with CD63+ or MHC-1+ EVs showed surface marker- and CD47-dependent changes in gene expression in the target cells. Gene set enrichment analysis identified CD47-dependent and surface marker-dependent effects of T cell EVs on VEGF and inflammatory signaling, cell cycle, and on lipid and cholesterol metabolism. Thus, subsets of T cell EVs differentially regulate endothelial cell metabolism and inflammatory and angiogenic responses. HUVEC (ATCC) were co-cultured with immunocaptured CD63+ and MHC-1+ EVs or control beads alone from Jurkat and JinB8 T cells for 3 days. The total RNA was extracted using miRNA easy kit equal amount of RNAs was subjected to global microarray analyses