Single cell analysis of sister blastomeres of 2-cell mouse embryos respecting pair associations

Upon 2-cell embryo splitting, individual blastomeres were compared and contrasted with each other respecting pair associations (e.g. blastomere '1a' and '1b' of embryo 1, '2a' and '2b' of embryo 2, and so forth) Transcriptome analysis followed by cluster analysis (Ward) was able to match a minority of the blastomeres with the correct sister blastomere Pair association (e.g. blastomere 'a' and 'b' from the same 2-cell embryo) was rigorously observed在对2细胞胚胎进行裂解操作后，研究人员基于配对关联规则对单个卵裂球（blastomere）进行两两比较，例如胚胎1的卵裂球"1a"与"1b"、胚胎2的卵裂球"2a"与"2b"，依此类推。随后开展转录组分析（Transcriptome analysis）并采用沃德（Ward）法进行聚类分析，仅能将少数卵裂球与其正确的姐妹卵裂球进行匹配。实验严格遵循配对关联原则（例如来自同一2细胞胚胎的卵裂球"a"与"b"）。