Transcriptional Regulation by NFATc1 during murine thymocyte development [gammadelta_T_cells RNA-seq]

In thymus hematopoietic precursor cells differentiate into aß T cells, ?d T cells, mucosa-associated invariant T cells (MAIT), and natural killer T (NKT) cells. We show that both ablation of NFATc1 or its induction during the DN stages of thymocyte development leads to an almost normal thymocyte development but a marked increase in ?d T cells. The ?d cells deficient for NFATc1 acquire an NKT ?d cell phenotype that exhibits the expression of CD4 co-receptor, the NK1.1 marker, the augmented usage of the V?1.1 and Vd6.3 segments, and an increased in IL4 and IFN-? production. Overall design: Transcriptom analysis of isolated ?d thymocytes. Nfatc1-fl/fl (WT) express normal levels of NFATc1 while in Rag1cre-Nfatc1-fl/fl NFATc1 expression is ablatedby Rag1 driven Cre activity (KO). For both settings triplicates were analysed