tissue-specific pattern of mRNA expression

The tissue-specific pattern of mRNA expression can indicate important clues about gene function. High-density oligonucleotide arrays offer the opportunity to examine patterns of gene expression on a genome scale. Toward this end, we have designed custom arrays that interrogate the expression of the vast majority of protein-encoding human and mouse genes and have used them to profile a panel of 79 human and 61 mouse tissues. The resulting data set provides the expression patterns for thousands of predicted genes, as well as known and poorly characterized genes, from mice and humans. We have explored this data set for global trends in gene expression, evaluated commonly used lines of evidence in gene prediction methodologies, and investigated patterns indicative of chromosomal organization of transcription. We describe hundreds of regions of correlated transcription and show that some are subject to both tissue and parental allele-specific expression, suggesting a link between spatial expression and imprinting. Keywords: different tissues baseline gene expression was taken from various human and mouse tissues信使RNA（mRNA）的组织特异性表达模式，可为基因功能研究提供重要线索。高密度寡核苷酸芯片（high-density oligonucleotide arrays）可实现全基因组范围内的基因表达模式检测。为此，我们设计了定制化寡核苷酸芯片，用以检测绝大多数蛋白编码的人类与小鼠基因的表达水平，并利用该芯片对由79种人类组织和61种小鼠组织组成的队列进行了表达谱分析。本数据集涵盖了小鼠与人类中数千个预测基因、已知基因以及功能注释匮乏的基因的表达模式。我们针对该数据集开展了多维度分析：探究基因表达的全局趋势，评估基因预测方法中常用的证据类型，并研究与转录过程染色体组织特征相关的表达模式。我们报道了数百个存在转录共调控的区域，并发现其中部分区域同时受组织特异性和亲本等位基因特异性表达调控，这提示空间表达模式与基因组印记之间存在关联。关键词：不同组织；基线基因表达（数据采自多种人类与小鼠组织）