A Comprehensive Analysis of the Lysine Acetylome Reveals Diverse Functions of Acetylated

Lysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced de-etiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during de-etiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 875 lysine acetylated sites on 494 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast and mitochondria. In addition