Short Peptides based on the conserved regions of MIEN1 protein exhibit anti-cancer activity by targeting the MIEN1 Signaling Pathway

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways in different types of cancers. The goals of this study are to identify differentially expressed genes(DEGs) upon MIEN1 derived anticancer peptide treatment and its analogs by RNA-seq and to compare quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Overall design: MDA-MB-231 cellular mRNA profiles of Peptide-treated and Untreated breast cancer cells