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Single-nucleus transcriptomics of inducible Ngly1-/- cerebellum

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295078
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To explore the cell type-specific effects of NGLY1 deficiency and the STING pathway in the cerebellum, we performed single-nucleus RNA sequencing (snRNA-seq) of cerebella from Ngly1fl/fl, iNgly1-/- and iNgly1-/-Sting1-/- mice Eight-week-old Ngly1fl/fl, iNgly1-/- and iNgly1-/-Sting1-/- male mice (2 mice/genotype) were sacrificed and cerebella were collected and frozen in liquid nitrogen. Nuclei were isolated from frozen cerebella and droplet-based snRNA-sequencing was performed using 10x Genomics.
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2025-07-31
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