Transcriptome data

Jack and three OE-GmAAP7 lines were grown in field at 2024 for transcriptome sequencing. A randomized block design was used for field trials, each accession was planted in a one-row plot, with each row 2 m long and 50-cm row spacing. Seed samples of six plants of each line were collected at 45 days after flowering, seed samples from each line were mixed and stored at -80 degree C freezer. Approximately 2 g seed tissue from each line was collected, and three biological replicates were used per line. Library construction was performed followed by sequencing on the Illumina NovaSeq 6000 at Shanghai Biozeron Biotech (Shanghai, China), producing 150-bp paired-end reads. An average of 6.7 Gb clean data was generated.