Genome wide mapping of open chromatin in senescence cells upon knockdown of senescence associated transcription factors

ATAC-seq was performed upon senescent BJ fibroblasts upon controls shRNA or knockdown of p65, SMAD2 or SMAD3, or NFI family members, in order to determine the role of each factor upon senescence associated enhancers. Overall design: Senescent BJ fibroblasts were collected after 2 weeks knockdown of listed transcription factors, and ATAC-seq was performed upon each sample. Knockdown samples were compared with control GFP knockdown, in terms of the ATAC-seq tag counts at senescence associated enhancers.