IRS2 Promotes Proliferation and Drives Invasion-Migration in SI-NETs.

I investigated the role of insulin receptor substrate 2 (IRS2) in small-intestinal neuroendocrine tumours (SI-NETs). Using SI-NET cell lines (STC-1 and Glu-tag), I found that IRS2 was significantly upregulated. IRS2 knockdown inhibited cellular proliferation, migration, and invasion, while its overexpression promoted these processes. For in vivo validation, I established stable IRS2-knockdown cell lines and performed xenograft experiments in nude mice. Transcriptomic profiling by RNA sequencing was conducted to identify downstream targets of IRS2 signaling. Overall design: RNA sequencing was performed on control and stable IRS2-knockdown SI-NET cell lines (Glu-tag) in biological triplicate to identify transcriptomic changes induced by IRS2 silencing.