Transcriptome analysis of jurkat cell effected by sorting

The enrichment of viable cells is an essential step in single-cell sequencing for clinical and biotechnology research applications. A high-end jet-in-air sorter is a fluorescent-activated cell sorting (FACS) sorter that ideally processes cells with high purity, yield and viability. However, the current sorting strategy has not achieved satisfactory performance to date. In this study, to achieve high-purity, high-yield, high-viability cell sorting, we explored three aspects. Moreover, the level of gene expression remained steady in the 5% FBS collecting buffer.