Results of Long template PCRs and regular PCRs screening for presence of the entire E. faecalis PAI.

Presence of the entire PAI was investigated based on reference strain MMH594 [1]) in the donor and transconjugant strains PAI overlapping regions were arbitrarly chosen to allow long-PCR amplification as shown in Table S1 and Figure 1. Recipient strains were also tested and were negative for all PCRs.* A product of unexpected size was amplified. an insertion within this region(s) is suspected. a deletion within this region(s) was confirmed.