Source Data for: "Modified QuEChERS with Florisil Cleanup for Organophosphorus Pesticide Analysis in Agricultural Soils by Gas Chromatography-Tandem Mass Spectrometry"

DATASET TITLE:Source Data for: "Modified QuEChERS with Florisil Cleanup for Organophosphorus Pesticide Analysis in Agricultural Soils by Gas Chromatography-Tandem Mass Spectrometry" AUTHORS:Julián David Beltrán-Ardila, Peter Alexander Escobar-Correa, Leidy Valentina Olaya-Bocanegra,Diana Angélica Varela-Martínez*, Miguel Ángel González-Curbelo(*Corresponding author)Departamento de Ciencias Básicas, Facultad de Ingeniería, Universidad EAN, Bogotá D.C., Colombia ASSOCIATED PUBLICATION:Journal: Journal of Visualized Experiments (JoVE)Manuscript ID: JoVE72745Status: Under revision DATASET DESCRIPTION:This dataset contains the source data underlying the figures and tables reported in the associated manuscript. All data were generated at the Laboratorios of Universidad EAN. FILES INCLUDED: 1. Figure 2_SourceData.xlsx - Co-extractive removal (%) by seven dSPE sorbent combinations across six agricultural soils - Determined gravimetrically (n=3 replicates) - Source data for Figure 2 in the manuscript 2. Figure 3_SourceData.xlsx - Matrix effect percentage (ME%) per analyte (12 OPPs) and soil (6 matrices) - Calculated as: ME (%) = [(slope_matrix / slope_solvent) − 1] × 100 - IS: chlorpyrifos-methyl-d10 (procedural IS) - Source data for Figure 3 (heatmap) in the manuscript 3. Table 2_SourceData.xlsx - Matrix-matched calibration parameters (slope, intercept, sy/x, R²) per analyte per soil - Matrix effect percentages per analyte per soil - Source data for Table 3 in the manuscript - Note: partial dataset shown (representative analytes); full dataset in manuscript Table 3 METHODS SUMMARY:- Extraction: Modified QuEChERS AOAC Method (ACN with 1% acetic acid, MgSO4/NaOAc)- Cleanup: Dispersive SPE with 150 mg MgSO4 + 50 mg PSA + 50 mg C18 + 50 mg Florisil - Instrument: Shimadzu GCMS-TQ8040 (GC-QqQ-MS/MS), MRM mode- Column: SH-Rxi-5Sil MS (30 m × 0.25 mm × 0.25 µm)- Validation: SANTE/2020/12830 (Rev. 2)- Target analytes: 12 organophosphorus pesticides (OPPs)- Calibration range: 5–600 µg/kg SOILS:S1 = Villavicencio, Meta, Colombia (467 m a.s.l., OM 0.18%, clay)S2 = Sabana de Bogotá, Cundinamarca, Colombia (2650 m a.s.l., OM 1.04%, clay loam)S3 = Floresta, Boyacá, Colombia (2506 m a.s.l., OM 1.71%, clay loam)S4 = Guasca, Cundinamarca, Colombia (2700 m a.s.l., OM 6.46%, loam)S5 = Santa Rosa de Viterbo, Boyacá, Colombia (2748 m a.s.l., OM 2.74%, sandy clay loam)S6 = La Laguna, Tenerife, Spain (549 m a.s.l., OM 1.27%, clay loam) FUNDING:Research and Transfer Management, Universidad EAN, Bogotá D.C., Colombia.