five

Lottia gigantea Raw sequence reads

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP496524
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Low coverage whole genome sequencing data for 19 populations, with roughly 30 individuals sampled per population. We used the Qiagen DNAeasy extraction kit to perform DNA extractions following manufacturer protocols. DNA quantity and quality were assessed with Nanodrop, Qubit, and gel electrophoresis. Library preparation followed the Nextera Lite protocol, with adaptations following Rowan et al. (2019). Briefly, this consisted of normalizing samples, running a Polymerase Chain Reaction (PCR), pooling samples, and bead purification and size selection. Libraries were sent to BGI Genomics for whole-genome sequencing on the DNBSEQ-T7 PE150 platform on four lanes of sequencing.
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2024-06-30
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