DNA methylation estimation using methylation-sensitive restriction enzyme bisulfite sequencing (MREBS). DNA methylation estimation using methylation-sensitive restriction enzyme bisulfite sequencing (MREBS)

We introduce methylation-sensitive restriction enzyme bisulfite sequencing (MREBS) which has the reduced sequencing requirements of RRBS, but significantly expands the coverage of CpG sites in the genome. Here, we compare MREBS to WGBS and RRBS. Overall design: Examine DNA Methylation in two cell lines using 3 different methods (MREBS, WGBS and RRBS)