MEF reprogramming

scRNA-seq time-series dataset of mouse embryonic fibroblast (MEF) reprogramming to induced endoderm progenitors (iEPs)assayed using Dropseq and 10x Genomics. Contains 8 time points spanning days 0-28 during reprogramming. Total cell number is 104,887.<br>This dataset contains raw spliced and unspliced counts estimated using the velocyto pipeline (La Manno et al., Nature 2018) that can be used to compute RNA velocity. Further, this dataset contains clonal information from CellTagging.<br>Please cite the original publication: https://doi.org/10.1038/s41586-018-0744-4

本数据集为单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）时间序列数据集，聚焦小鼠胚胎成纤维细胞（mouse embryonic fibroblast, MEF）重编程为诱导内胚层祖细胞（induced endoderm progenitors, iEPs）的过程，采用Dropseq与10x Genomics技术完成检测。该数据集涵盖重编程过程中0至28天的8个时间节点，总细胞数达104887个。
本数据集包含通过velocyto分析流程（La Manno等，《Nature》2018年）估算得到的原始剪接计数与未剪接计数数据，可用于计算RNA速率（RNA velocity）。此外，数据集还包含来自CellTagging技术的克隆信息。
请引用该原始文献：https://doi.org/10.1038/s41586-018-0744-4