NGS data related to Rajaram et al.: Allele specific DNA demethylation ...
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Method overview To achieve targeted locus and allele-specific DNA demethylation, HEK293 cells were transfected with two plasmids. One plasmid contains, dCas9 fused to a SunTag...
方法概述 为实现靶向位点与等位基因特异性DNA去甲基化，将人胚肾293细胞（HEK293）用两种质粒进行转染。其中一种质粒包含与SunTag融合的失活CRISPR相关蛋白9（dCas9）……
