Figure 2 qPCR.pzfx

Fig. 2 Expression analysis of epithelial markers by RT-qPCR of the different cellular models and respiratory mucosa from trachea for comparison. Expression levels were referenced to GAPDH as housekeeping gene and are expressed as ∆c_p with low values for a strong expression of the marker and high values for a weak expression of the indicated mRNA (see color scale on the right side). Relevant markers for different mucosal cell types as indicated on the left side were analyzed, but also expression of Fc receptors since they are relevant for IgG uptake, distribution, and elimination in airway mucosa. Values are shown as mean. Trachea tissue: n = 6 - 12, N = 2 – 4; TMPC Flask method: n = 9 - 12, N = 3 - 4, TMPC DS: n = 6 - 12, N = 2 – 4; RPMI 2650: n = 4 - 12, N = 1 - 3; CaLu3: n = 4 - 12, N = 1 - 3.