Transcriptomes of iPSC-derived and post-mortum Hypothalamus Neurons from obese and control donors

We sequenced the transcriptomes of seven samples of hypothalamic neurons dervied from pluripotent stem cells taken from healthy patients; five samples of hypothalamic neurons derived from pluripotent stem cells of constitutionally obese donors; five samples of sectioned hypothalami from post-mortem dissection of brains, and two samples for motor neurons derived from pluripotent stem cells of healthy donors in order to assess the similarity of our iPSC-derived cells against those of sectioned brains and to identify possible transcriptional disfunction which might underlie extreme, inherited obesity. Overall design: We sequenced mRNA collected from post-mortem brains and from samples generated by differentiating pluripotent stem cells into hypothalamic neurons and motor neurons.