Chip-on-ChEPseq analysis of RAD21 cohesin subunit cleaved by separase in a human cancer cell line

The goal of the study was to map the epigenomic positions of proteolyzed RAD21 human protein in mitotic DLD-1 cells with proprietary antibodies against the neo-epitope (RAD21*) generated by cleavage with separase. The study involved chromatin IP analyzed by high-throughput sequencing analysis using immortalized human cancer cells. DLD-1 (ATCC® CCL-221™) cells were grown at standard conditions and synchronized using double thymidine block protocol. The cells synchronously progressing through mitosis were collected at equal intervals in order to capture anaphase cells.