Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture [Bisulfite-Seq]

To further reveal the major cell types of developing pIVC embryos and underlying epigenetic dynamics, the optimized single-cell based multi-omics sequencing method scChaRM-seq was performed (Yan et al., 2021b). 1,862 single cells Bisulfite-seq datasets were further analyzed. We then performed multi-omics profiling analysis using data obtained from9 pIVC embryos at 8 sequential developmental stages. Overall design: Methylation profiling by high throughput sequencing