Temporal dynamic reorganization of 3D chromatin in hormone-induced breast cancer and endocrine resistance

To establish a data-driven learning model of the temporal dynamics and 3D chromatin reorganization, we conducted tethered chromatin conformation (TCC) sequencing to examine 3D structure dynamics in estradiol (E2)-induced breast cancer MCF7 cells and Tamoxifen resistant breast cancer MCF7 cells. Overall design: In this study, we conducted TCC analysis in hormone-starved MCF7 cells (T0) and compared this to MCF7 cells treated with 1hr of E2 (T1), 4hr of E2 (T4), 16hr of E2 (T16), 24hr of E2 (T24) and Tamoxifen resistant MCF7 cells. We integrated next generation 3D chromatin sequencing data with ChIP-seq data of histone marks H3K4me1, H3K4me3, H3K27ac, H3K9me3, and H3K27me3 and gene expression data.