Data and code for: Recombinant venom proteins in insect seminal fluid reduces female lifespan

Publication: https://doi.org/10.1101/2024.01.14.575309

This repository contains all the data as well as the scripts used for the analysis and visualisation. 

File
Description

TMT.ipynb
The Jupyter notebook containing the Python code for statistical analysis and figure generation. Scripts are sectioned in the order that results are presented in the study, with sub-headings and figure numbers where appropriate.

actingal4.csv
Offspring phenotype data from the UAS:venom x Act5C-GAL4 assay (Results: Testing functional expression of recombinant venoms).

femalelifespan_1-1.csv
Female lifespan data from the 1:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored. 

femalelifespan_3-1.csv
Female lifespan data from the 3:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored. 

singlemating.csv
Data from the single-pair courtship assay. 1 = successful courtship observed; 0 = successful courtship not observed.

competitivemating.csv
Offspring genotype data from the competitive mating assay. Female = group of offspring from a given mother. white = positive result from gDNA PCR using the 'white' primers (supplementary table S2). TMT = positive result from gDNA PCR using the 'UAS' primers (supplementary table S2).

malelifespan.csv
TMT male lifespan data from the male longevity assay. Time = days since eclosion. Status: 1 = death observed; 0 = censored. 

TMT-GAMA.db and TMT-lethality.db contains data from the GAMA model (doi:10.5281/zenodo.11439089), which is accessed by the Jupyter notebook.

Table
Description

TMT_step_output
Each simulation will write key values to this table each cycle once transgenic males have begun to be released.

TMT_female_output
Each female will write key values to this table upon their death once transgeni males have begun to be released.

TMT_PR50
Each simulation will write to this table when/if the female population reaches 50% of its initial size.

TMT_PR95
Each simulation will write to this table when/if the female population reaches 95% of its initial size.