Characteristics of MAbs against the NS1 protein of WNV.

aPurified recombinant WNV-NS1 protein and DENV-NS1 were used as the coating antigen and were reacted with purified MAbs against the NS1 protein. The absorbance was measured at 450 nm: +, OD450 = 0.5 to 1; ++, OD450 = 1 to 2; +++, OD450 = 2 to 3; OD450>3. bThe reactivity of each MAb was determined by IFA with WNV, DENVs, YFV, and JEV, and their IFA reactivity with normal cells were negative (data not shown). −, no reactivity; +, weak reactivity; ++, intermediate reactivity; +++, strong reactivity. cNot detected. Characteristics of MAbs against the NS1 protein of WNV.