Modulation of gene expression by rapamycin in hepatic cell lines, WB-F344 and WB311

Two rat hepatic cell lines, WB-F344 and WB311, were characterized for the effect of rapamycin on gene expression. The WB311 cell line, which is tumorigenic and resistant to the growth inhibitory effects of rapamycin, was originally derived from the WB-F344 parental hepatic epithelial cell line. The goal of this experiment was to identify genes that responded to rapamycin in the sensitive cells but not the resistant cells, thereby providing insight into the mechanism of rapamycin resistance.为探究雷帕霉素（rapamycin）对基因表达的影响，本研究对两种大鼠肝细胞系WB-F344与WB311开展了表征分析。其中WB311细胞系具有致瘤性，且对雷帕霉素的生长抑制效应产生耐药性，其最初源自亲本肝上皮细胞系WB-F344。本实验的核心目标为筛选出仅在雷帕霉素敏感细胞中呈现表达响应、但耐药细胞中无对应响应的基因，从而为阐明雷帕霉素耐药机制提供新的研究视角。