Changes in gilthead seabream (Sparus aurata) gill glycosylation upon Sparicotyle chrysophrii infection

Study of the changes in gilthead seabream gill glycosylation upon S. chrysophrii infection using liquid chromatography mass spectrometry (LC/MS). The results were correlated against expression levels of gill glycotransferases, the number of S. chrysophrii parasites, the abundance of 2013Ark19i bacteria in the gill microbiota as well as abundance of acidophilic granulocytes and goblet cells in the gill tissue to contribute to the understanding of this disease and associated problems.

The file Fish data.xlsx includes the individual information of the parasite abundance, weight, goblet cell and acidophilic granulocyte quantification, and percentage of abundance of 2013Ark19i in the gill microbiota. 

The file Supplementary File 1_Glycan quantification.xlsx includes the individual values of all indetified glycan structures.

The file Transferase data.xlsx includes the individual expression levels of the 132 enzymes related to glycosylation identified in the gilthead seabream genome.

The file Supplementary File 2-Differentially expressed transcripts related toglycosylation.pdf shows a bar graph with the expression of the differentially expressed transcripts (P < 0.05)

The file Transcripts statistics.pzfx shows the statistical analysis performed to detect the differentially expressed transcripts related to glycosylation showed in Supplementary File 2. 

The file Correlations.pzfx shows the correlation analyses performed to find associations between glycome structures and parasite, microbiota, acidophilic granulocytes or goblet cells abundance. 

The file Stats on core and terminal structures.pzfx contains the statistics performed on the detected core and terminal glycan structures.

The file Stats on individual O and N glycans.pzfx contains the statistics performed comparing the relative abundance of all detected O- and N-glycans between control and infected fish.